Print  
 
24hr DNA Sequencing
Oligonucleotides
Company Info
Staff Information
Technologies 
Ordering & Shipping
Catalog Numbers
Customer Service
Home
 
 

Shipping Information

In order to help us provide the best possible service, please read and follow the subsequent instructions on submitting samples to SeqWright. Customers may also download our supplementary SHIPPING INFORMATION PAGE (MS WORD format) for additional information.

Project Description Form:

A completed Project Description Form must be included with all project sample submissions to SeqWright. The form has fields for contact and billing information, services requested, and sample information, which should all be filled out by the customer. The form also asks for data retrieval preferences as well as special requirements needed for completing the project. It is very important that this form is filled out completely and correctly in order to assure that a project is completed as intended by the investigator.

Customers must provide either a purchase order number or credit card number on the completed Project Description Form. Not providing this information may lead to delays in completing the project.

Project Description Forms are available for download, or one may be completed online by clicking on the link below. Submitting a Project Description Form online may result in faster processing and turnaround of the project.

DOWNLOADABLE PROJECT ORDER FORM (Word Format)

ONLINE PROJECT ORDER FORM

ONLINE OLIGO ORDERING

DNA Preparation:

DNA should be purified by column or gel purification methodologies. Any of the commercially available column purification systems will produce DNA of sufficient quality for sequencing. Please indicate on the Project Description Form whether or not the DNA has been purified, and if so, list the method/kit used.

Sample Concentration:

Please refer to the following chart for submitting samples:

Template
Type
Template Concentration Template Volume Custom Primer Concentration Custom Primer Volume
PCR 30 ng/µl 10 µl/reaction 3.2 µM 10 µl/reaction
Plasmid 0.2 µg/µl 10 µl/reaction 3.2 µM 10 µl/reaction
Cosmid, BAC 1 µg/µl 10 µl/reaction 10 µM 10 µl/reaction
Genomic DNA 1 µg/µl 10 µl/reaction 10 µM 10 µl/reaction

 

Submitting 96- & 384-well Plates:

Please fill out the sample information fields of the Project Description Form as outlined below. DNA samples and primers must be submitted in separate plates. Samples must be arranged descending vertically (A1, B1...) as shown in the diagram below.

Shotgun Library Constructions:

For custom shotgun library construction or for shotgun sequencing services, the customer must provide either 100µg of purified DNA or a clone from which to grow and purify DNA. DNA should be prepared either by commercial column methods or by CsCl gradient. Warning: Contamination of plasmid or BAC DNA by genomic DNA will make sequencing of the library very difficult and can necessitate the reconstruction of a library.

Shipping Instructions:

All samples (tubes/plates) should be properly sealed and shipped in shock-resistant packaging in order to prevent damage during shipment. Purified DNA should be sent in either TE or water and may be shipped at room temperature. Plates and stabs may also be sent at RT. Glycerol stock cultures should be sent on dry ice.

Please send all sample submissions to the following address:

SeqWright, Inc.
2575 W. Bellfort #2001
Houston, TX 77054

To speak to a representative, please call 1-866-720-GENE (4363) or email custsrv@fishersequencing.com.

 

© SeqWright, Inc. 2004.