Primer walk sequencing is the method of choice for sequencing DNA fragments between 1.3 and 7 kb. The DNA of interest may be either a plasmid insert or a PCR product. The initial sequencing will be performed from each end using either universal primers or primers designated by the customer. In order to completely sequence the region of interest SeqWright will design and synthesize primers as necessary to obtain contiguous sequence information.

Single Strand Primer Walks:

Single strand primer walks provide contiguous sequence information for one strand of the region of interest. Due to the fact that only one strand is sequenced we can only guarantee an accuracy level of between 95 and 98 percent. The customer will receive the edited consensus sequence as well as the contig map of all sequencing performed to cover the region. The turnaround time for this service can be estimated at approximately 1.5 kb/Week.

Double Strand Primer Walks:

 

Pricing and turnaround times differ with template types and project sizes. Discount pricing is available for large volume orders. Please contact our Sales Department for more information on this and other services.